Coding

Part:BBa_K1072004

Designed by: Zewei yan, Yichong wang and Junjie zhang   Group: iGEM13_SCUT   (2013-09-07)

alpha-ALS Coding Region

BBa_K1072004 is the conding region of α-acetolactate synthetase(α-ALS).

The synthetize pathway of diacetyl, in E.coli, beginning with the central pathway metabolite pyruvate as the precursor molecule, the first step in the diacetyl biosynthetic pathway involves the condensation of two molecules of pyruvate to yield one molecule of acetolactate, and is catalyzed by acetolactate synthase (ALS).The stability of acetolactate is known to be affected by the presence of oxygen, which causes its spontaneous decarboxylation to produce diacetyl,figure 1.

BBa_K1072004 was inserted into PET22b(+) and expressed successfully in E.coli(BBa_K1072005).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//direction/forward
transcriptional
Parameters
proteinalpha-ALS